Iimpawu zeMpilo yeCanine ezidityanisiweyo zokuFumana (izinto ezi-5-6)

【 Injongo yovavanyo 】
I-Canine pancreatic lipase (cPL) : I-Canine pancreatitis sisifo sokuvuvukala kwe-pancreas. Ngokuqhelekileyo, inokwahlulwa ibe yi-pancreatitis ebukhali kunye ne-pancreatitis engapheliyo. I-pancreatic neutrophil infiltration, i-pancreatic necrosis, i-peripancreatic fat necrosis, i-edema kunye nokulimala kunokubonwa kwi-pancreatitis enzima. I-Pancreatic fibrosis kunye ne-atrophy inokubonwa kwi-pancreatitis engapheliyo. Xa kuthelekiswa ne-pancreatitis ebukhali, i-pancreatitis engapheliyo ayinabungozi kangako, kodwa ihlala rhoqo. Xa izinja zihlushwa yi-pancreatitis, i-pancreas yonakaliswa, kwaye inqanaba le-pancreatic lipase egazini landa ngokukhawuleza. Okwangoku, i-pancreatic lipase sesinye sezona zibonakaliso zibalaseleyo zokuxilongwa kwe-pancreatitis kwizinja.
I-Cholyglycine (CG) yenye ye-conjugated cholic acid eyenziwe ngokudityaniswa kwe-cholic acid kunye ne-glycine. I-Glycocholic acid yeyona nxalenye ibalulekileyo ye-bile acid kwiserum ngexesha lokukhulelwa kade. Xa iiseli zesibindi zonakaliswe, ukunyuswa kweCG ngamaseli esibindi kuncipha, okubangele ukwanda komxholo weCG egazini. Kwi-cholestasis, ukukhutshwa kwe-cholic acid ngesibindi kuphazamisekile, kwaye umxholo weCG ubuyele kumjikelezo wegazi uyanda, okwandisa umxholo weCG egazini.
I-Cystatin C yenye yeeprotheni ze-cystatin. Ukuza kuthi ga ngoku, iCys C yinto engapheliyo ehlangabezana neemfuno zemakishi egqibeleleyo yeGFR. Isalathiso esibucayi kunye nesicacileyo sovavanyo lomsebenzi we-canine renal.
I-N-terminal pro-brain natriuretic peptide (Canine NT-proBNP) yinto efihliweyo yi-cardiomyocytes kwi-Canine ventricle kwaye ingasetyenziselwa njengesalathisi sokufumanisa ukuhluleka kwentliziyo ehambelanayo. Ukuxinwa kwe-cNT-proBNP egazini kuhambelana nobunzima besifo. Ngoko ke, i-NT-proBNP ayikwazi nje ukuvavanya ubunzima bokungaphumeleli kwentliziyo ebukhali kunye nengapheliyo, kodwa isetyenziswe njengesalathisi se-prognosis yayo.
I-Canine allergen iyonke i-IgE (cTIgE) :I-IgE luhlobo lwe-immunoglobulin (Ig) enobunzima bemolekyuli ye-188kD kunye nomxholo ophantsi kakhulu kwi-serum. Ngokuqhelekileyo isetyenziselwa ukuxilongwa kweempendulo ze-allergies. Ukongeza, inokunceda ekuxilongeni usulelo lwe-parasitic kunye ne-myeloma eninzi. 1. I-Allergic reaction: xa i-allergic reaction isenzeka, ikhokelela ekwandeni kwe-allergen ye-lgE. Xa iphezulu i-lgE ye-allergen, kokukhona i-aleji iyingozi ngakumbi. 2. Usulelo lwe-Parasite: emva kokuba isilwanyana sasekhaya sosulelwe zizifunxi-gazi, i-allergen ye-lgE inokunyuka, ngokuqhelekileyo inxulumene nokwaliwa okuncinci okubangelwa yi-parasite proteins. Ukongeza, ubukho obuxeliweyo bomhlaza bunokuba negalelo ekunyusweni kwe-IgE epheleleyo.

【Umgaqo-siseko wokufunyanwa】
Le mveliso isebenzisa i-fluorescence immunochromatography ukufumanisa ngokobungakanani i-cPL/CG/cCysC/cNT-proBNP/cTIgE umxholo kwigazi le-canine. Umgaqo osisiseko kukuba i-nitrocellulose membrane iphawulwe ngemigca ye-T kunye ne-C, kwaye umgca we-T uhlanganiswe ne-antibody a eqaphela ngokukodwa i-antigen. Iphedi yokubopha ifafazwa ngenye i-fluorescent nanomaterial ebhalwe i-antibody b enokwazi ngokuthe ngqo i-antigen. I-antibody kwisampulu ibophelela kwi-nanomaterial ebhalwe i-antibody b ukuze yenze i-complex, ethi emva koko ibophelele kwi-antibody ye-T-line ukuze yenze isangweji isakhiwo. Xa ukukhanya okuvuyisayo kukhanya, i-nanomaterial ikhupha iimpawu ze-fluorescent. Ukuqina komqondiso kwakuhambelana ngokufanelekileyo kunye noxinzelelo lwe-antigen kwisampuli.