Ukuxinwa kwe-progesterone ye-canine kwi-serum ihambelana nenqanaba le-estrus yenja.Xa kuthelekiswa ne-LH, i-concentration ye-cProg isoloko ikhula ngexesha le-estrus yenja yemazi, ekulula ukuyilandelela kwaye inokuguqulwa ngexesha langempela Ixesha elifanelekileyo lokuzala liyi-3-6 iintsuku emva kokunyuka kwe-LH, kuxhomekeke kumfazi. imeko ye-estrus yenja.Phakathi kwabasetyhini abahlukeneyo, Inqanaba leprogesterone elihambelana nexesha elifanelekileyo lokukhwela liye lahluka ngokubanzi, ngokubanzi ukusuka kwi-0-50ng / ml, kodwa kwakukho ngaphezu koko Kolu luhlu, ngoko ke, iqondo le-keratinization ye-epithelium yangasese yadityaniswa Uhlolo oluguqukayo lwexesha lokwenyani lokugxilwa kwe-serum progesterone Indlela yokuvavanya inokuphucula kakhulu ukuba nokwenzeka kokukhawulwa kwezinja ezibhinqileyo.
Umxholo we-cProg kwi-serum yenja/i-plasma yafunyanwa ngokobungakanani nge-fluorescence immunochromatography.Umgaqo osisiseko: I-Fiber nitrate Kukho imigca ye-T kunye ne-C kwifilimu ene-dimensional ngokulandelanayo, kwaye umgca we-T uqatywe nge-cProg antigen a, enokwazi ngokuthe ngqo i-cProg ngokutshiza kwi-pad.
I-cProg kwisampulu yaqala yadityaniswa ne-nanomaterial ebhalwe i-antibody b ukwenza i-Complex, kwaye emva koko kwinqanaba eliphezulu, i-complex ikhuphisana ne-T-line antigen a kwaye ayinakubanjwa;Endaweni yoko, xa kungekho sampuli Phambi kwe-cProg, i-antibody b ibophelela kwi-antigen a.Xa i-excitation light irradiation, i-nanomaterial ikhupha isignali ye-fluorescence.Amandla omqondiso ahambelana ngokuphambeneyo noxinaniso lwe-cProg kwisampulu.
Kuba amanqanaba afanelekileyo eprogesterone anxulumene nokuzala, ubudala kunye nobukhulu benja, akukho xabiso lisisigxina, olu luhlu lulandelayo.
Ngokubhekiselele kuphela, kucetyiswa ukuba ilabhoratri nganye okanye isibhedlele ngasinye simisele uluhlu lwaso lwezalathiso ngokwekliniki
Akukho kubushushu:<1ng/ml;
Estrus:ukuxinwa kweprogesterone kwandisa ngokuthe ngcembe, umjikelezo ngokuqhelekileyo iintsuku ezi-7-8;Ukuze kuphuculwe izinga lokuphumelela lokukhulelwa, uvavanyo lokuqala lweprogesterone
Ugxininiso kufuneka lube phakathi kwe-10-50ng / ml, kwaye kucetyiswa ukuzala kabini.
10-30ng/ml:okokuqala ukukhwelana phakathi kwe-3h, ukukhwelana okwesibini ngaphakathi kwe-48h;
30-60ng/ml:okokuqala ukukhwelana phakathi kwe-2h kunye nokukhwelana okwesibini ngaphakathi kwe-24h;
60-80ng/ml:2h yokukhwelana.
Uluhlu lokufumanisa le kit yi-1-80ng / ml.Ukuba idlula uluhlu, khawuleza <1ng/ml, okanye> 80 ng/ml.
Ukusukela ekusekweni kwayo, umzi-mveliso wethu uye waphuhlisa iimveliso zodidi lwehlabathi ngokubambelela kumgaqo
yomgangatho kuqala.Iimveliso zethu ziye zafumana igama elihle kushishino kunye nokuthembeka okuxabisekileyo phakathi kwabathengi abatsha nabadala..