I-T4 iyona mveliso ephambili yokukhuselwa kwe-thyroid, kwaye ikwayinxalenye engenakuhlawuleka yengqibelelo ye-hypothalamic-anterior pituitary-thyroid regulatory system.Yandisa izinga le-basal metabolic kwaye idlala indima ebalulekileyo ekuphuhliseni zonke iiseli zomzimba.I-T4 igcinwe kwi-follicle ye-thyroid ngokudibanisa ne-thyroglobulin, kwaye ifihliwe kwaye ikhutshwe phantsi kolawulo lwe-TSH.Ngaphezu kwe-99% ye-T4 kwi-serum ikhona ngendlela yokubopha kwezinye iiprotheni.Ukuvavanyelwa i-T4 iyonke kwisampulu yegazi kunokuxelela ukuba i-thyroid gland yakho isebenza ngokungaqhelekanga.
Le mveliso isebenzisa i-fluorescence immunochromatography ukufumanisa ngokobungakanani umxholo we-cTT4 kwi-dog serum/plasma.Umgaqo osisiseko: Imigca ye-T kunye ne-C iphawulwe kwi-membrane ye-nitrocellulose, umgca we-T uhlanganiswe ne-cTT4 antigen a, kwaye i-pad yokubopha ifafazwe nge-fluorescent nanomaterial ebhalwe i-antibody b ekwazi ukuqaphela ngokukodwa i-cTT4.I-cTT4 kwisampulu ibhalwe kuqala nge-nanomaterial.I-antibody b ibophelela ukwenza i-complex, kwaye emva koko i-chromatographs ukuya phezulu.I-complex ikhuphisana ne-T-line antigen a kwaye ayinakubanjwa;Ngokuchasene noko, xa kungekho cTT4 kwisampulu, i-antibody b ibophelela kwi-antigen a.Xa isibane sokuvuselela sikhanyisa, i-nano ikhupha isignali ye-fluorescent, kwaye amandla omqondiso aphikisana ngokuhambelana nokuxinwa kwe-cTT4 kwisampuli.
Ukusukela ekusekweni kwayo, umzi-mveliso wethu uye waphuhlisa iimveliso zodidi lwehlabathi ngokubambelela kumgaqo
yomgangatho kuqala.Iimveliso zethu ziye zafumana igama elihle kushishino kunye nokuthembeka okuxabisekileyo phakathi kwabathengi abatsha nabadala..