I-Feline Health Marker eDityanisiweyo yokuFumana (izinto ezi-5-6)

【 Injongo yovavanyo 】
I-Feline pancreatic lipase (fPL) : I-pancreas yi-pancreas yesibini ngobukhulu kwi-digestive gland kumzimba wesilwanyana (eyokuqala isibindi), esiphambi kwesisu somzimba, esahlulwe kwi-lobes yasekhohlo nasekunene. Umsebenzi wayo ophambili kukwenza ii-enzymes eziyimfuneko kumzimba.I-Pancreatitis yahlulwe kwi-pancreatitis ebukhali kunye ne-pancreatitis engapheliyo. Umonakalo obangelwa yinto yangaphambili ubuninzi bexeshana, ngelixa lo mva ushiya i-fibrosis engapheliyo kunye ne-atrophy ngexesha lokuvuvukala okungapheliyo. Phakathi kwabo, i-pancreatitis engapheliyo ibalelwa malunga ne-2/3 ye-cat pancreatitis.
I-Cholyglycine (CG) yenye ye-conjugated cholic acid eyenziwe ngokudityaniswa kwe-cholic acid kunye ne-glycine. I-Glycocholic acid yeyona nxalenye ibalulekileyo ye-bile acid kwiserum ngexesha lokukhulelwa kade. Xa iiseli zesibindi zonakaliswe, ukunyuswa kweCG ngamaseli esibindi kuncipha, okubangele ukwanda komxholo weCG egazini. Kwi-cholestasis, ukuphuma kwe-cholic acid ngesibindi kuphazamisekile, kwaye umxholo we-CG ubuyele kwi-circulation yegazi uyanda, okwandisa umxholo we-CG egazini. ngombhobho we-hepatic emva kokutya. Ngokufanayo, izifo zesibindi kunye nokuphazamiseka kwe-bile duct kunokubangela isalathisi esingaqhelekanga.
I-Cystatin C yenye yeeprotheni ze-cystatin. Umsebenzi obaluleke kakhulu we-physiological kukulawula umsebenzi we-cysteine ​​​​protease, enempembelelo enamandla kakhulu yokuthintela kwi-cathepsin B, i-papain, i-figs protease, kunye ne-cathepsin H kwaye ndakhululwa yi-lysosomes. Idlala indima ebalulekileyo kwimetabolism ye-intracellular peptides kunye neeproteni, ngakumbi kwi-metabolism ye-collagen, enokuthi i-hydrolyze ezinye ii-prehormones kwaye zizikhulule kwizicubu ekujoliswe kuzo ukuze zidlale indima yazo yebhayoloji. I-Hereditary cerebral hemorrhage kunye ne-amyloidosis sisifo esihambelana ngqo ne-cystatin C gene mutation, enokubangela ukuphuka kwe-cerebral vascular rupture, i-cerebral hemorrhage kunye nezinye iziphumo ezibi. Intso yindawo yodwa yokucima i-cystatin C ejikelezayo, kunye nokuveliswa kwe-cystatin C rhoqo. Izinga le-serum cystatin C ikakhulu lixhomekeke kwi-GFR, eyona nto ilungileyo i-endogenous marker ukubonisa utshintsho lwe-GFR. Utshintsho kumxholo wolunye ulwelo lomzimba lukwanxulunyaniswa neentlobo ngeentlobo zezifo.
I-NT-proBNP (i-N-terminal pro-brain natriuretic peptide), ekwaziwa ngokuba yi-B-type diuretic peptide, yihomoni yeprotheyini ekhutshwa yi-cardiomyocytes kwiiventricles zentliziyo. Xa i-ventricular blood pressure inyuka, i-ventricular dilation, i-myocardial hypertrophy, okanye uxinzelelo kwi-myocardium yanda, i-precursor ye-NT-proBNP, i-proBNP (equlethwe i-108 amino acids), ifihliwe kwigazi nge-cardiomyocytes.
I-Cat allergen iyonke i-IgE (fTIgE) :I-IgE luhlobo lwe-immunoglobulin (Ig) enobunzima bemolekyuli ye-188kD kunye nomxholo ophantsi kakhulu kwi-serum. Ngokuqhelekileyo isetyenziselwa ukuxilongwa kweempendulo ze-allergies. Ukongeza, inokunceda ekuxilongeni usulelo lwe-parasitic kunye ne-myeloma eninzi. 1. I-Allergic reaction: xa i-allergic reaction isenzeka, ikhokelela ekwandeni kwe-allergen ye-lgE. Xa iphezulu i-lgE ye-allergen, kokukhona i-aleji iyingozi ngakumbi. 2. Usulelo lwe-Parasite: emva kokuba isilwanyana sasekhaya sosulelwe zizifunxi-gazi, i-allergen ye-lgE inokunyuka, ngokuqhelekileyo inxulumene nokwaliwa okuncinci okubangelwa yi-parasite proteins. Ukongeza, ubukho obuxeliweyo bomhlaza bunokuba negalelo ekunyusweni kwe-IgE epheleleyo.
【Umgaqo-siseko wokufunyanwa】
Le mveliso isebenzisa i-fluorescence immunochromatography ukufumanisa ngokobungakanani umxholo we-fPL/CG/fCysC/fNT-proBNP/fTIgE kwigazi lekati. Umgaqo osisiseko kukuba i-nitrocellulose membrane iphawulwe ngemigca ye-T kunye ne-C, kwaye umgca we-T uhlanganiswe ne-antibody a eqaphela ngokukodwa i-antigen. Iphedi yokubopha ifafazwa ngenye i-fluorescent nanomaterial ebhalwe i-antibody b enokwazi ngokuthe ngqo i-antigen. I-antibody kwisampulu ibophelela kwi-nanomaterial ebhalwe i-antibody b ukuze yenze i-complex, ethi emva koko ibophelele kwi-antibody ye-T-line ukuze yenze isangweji isakhiwo. Xa ukukhanya okuvuyisayo kukhanya, i-nanomaterial ikhupha iimpawu ze-fluorescent. Ukuqina komqondiso kwakuhambelana ngokufanelekileyo kunye noxinzelelo lwe-antigen kwisampuli.