I-Canine Distemper Virus (CDV) iyinxalenye yentsholongwane ye-Measles yentsapho ye-Paramucosal virus, enokubangela ukusasazeka kwezifo ezithintekayo ze-canine (i-canine distemper) kwaye zikhokelela kwiziganeko zeklinikhi ezifana ne-conjunctivitis, inyumoniya kunye ne-gastroenteritis kwizinja, njl. Intsholongwane ye-distemper ibonakala ngokusweleka okuphezulu, ukosuleleka okunamandla kunye nekhondo elifutshane lokugula.Ngokukodwa phakathi kweenjana, kukho izinga eliphezulu losulelo kunye nokufa.
I-Canine adenovirus uhlobo lwe-II lunokubangela i-laryngotracheitis eyosulelayo kunye neempawu ze-pneumonia ezinjeni.Iimpawu zeklinikhi ziquka umkhuhlane ongapheliyo, ukhohlokhohlo, i-serous to mucinous rhinorrhea, i-tonsillitis, i-laryngotracheitis, kunye nenyumoniya.Ukususela kwizibalo zeziganeko zeklinikhi, isifo sixhaphake kakhulu kwiintsana ezingaphantsi kweenyanga ezi-4.Inkunkuma - okanye ukhohlokhohlo lonke lweqela lunokubangelwa kwintshontsho, ngoko ke isifo sibizwa ngokuba yi "kennel cough" ngokweempawu zeklinikhi.
Umkhuhlane weCanine ubangelwa ikakhulu ziintlobo zentsholongwane yomkhuhlane A ikakhulu i-H3N8 kunye ne-H3N2.Iimpawu zokuqala zifana kakhulu ne-bronchitis ye-kennel.Iqala ngokhohlokhohlo oluzingileyo olunokuhlala ukuya kutsho kwiiveki ezintathu kwaye lukhatshwa kukuphuma kwempumlo emthubi.
Ukufunyaniswa okuthembekileyo kunye nokusebenzayo kunendima ekhokelayo efanelekileyo ekuthinteleni kunye nokuxilongwa kunye nonyango.
Imveliso yayisetyenziselwa ukufumanisa ubungakanani be-CDV / CAV-2 / FluA Ag kwiliso le-canine, impumlo kunye nokukhutshwa komlomo nge-fluorescence immunochromatography.Umgaqo osisiseko: I-nitro fiber membrane iphawulwe ngemigca ye-T kunye ne-C ngokulandelanayo, kwaye imigca ye-T ifakwe kwi-antibodies a1, i-a2 kunye ne-a3 eqaphela ngokukodwa i-CDV / CAV-2 / i-FluA antigens.Ama-antibodies b1, b2 kunye ne-b3 abhalwe ngenye i-nanomaterial ye-fluorescent ekwazi ukuqaphela ngokukodwa i-CDV/CAV-2/FluA yafafazwa kwi-binding pad.I-CDV/CAV-2/FluA kwisampulu yokuqala idityaniswe ne-nanomaterial ebhalwe i-antibodies b1, b2 kunye ne-b3 ukwenza i-complex, kwaye emva koko waya kwinqanaba eliphezulu.I-complex idibene ne-T-line antibodies a1, a2 kunye ne-a3 ukwenza isakhiwo sesandwich.Xa ukukhanya okuvuthayo kugxininiswe, i-nanomaterial ikhupha isignali ye-fluorescence, kwaye amandla omqondiso ahambelana ngokufanelekileyo kunye nokuxhomekeka kwentsholongwane exhomekeke kwisampuli.
Ukusukela ekusekweni kwayo, umzi-mveliso wethu uye waphuhlisa iimveliso zodidi lwehlabathi ngokubambelela kumgaqo
yomgangatho kuqala.Iimveliso zethu ziye zafumana igama elihle kushishino kunye nokuthembeka okuxabisekileyo phakathi kwabathengi abatsha nabadala..