I-Canine iyonke ye-lgE yeKit yoBubungakanani (i-Fluorescent Immunochromatography Assay ye-Rare Earth Nanocrystals) (cTIgE)

[Igama lemveliso]

Igama: cTIgE uvavanyo lwenyathelo elinye

 

[Iinkcukacha zokuPakisha]

Iimvavanyo ezili-10/ibhokisi


Iinkcukacha zeMveliso

Iithegi zeMveliso

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Iimpawu zeklinikhi

I-Allergic reaction, ekwabizwa ngokuba yi-allergic reaction, kukusabela okungaqhelekanga komzimba kwinto enye okanye ngaphezulu, ukusabela okwaliwayo kunokwenzeka kwiintlobo ezahlukeneyo zezinja, ikliniki ixhaphake kakhulu kwizinja ezincinci, ezona mpawu ziphambili zezinja ezigulayo zezi: intloko, iinkophe ngeenxa zonke, i-edema yomlomo, i-hypoabdominal skin flush okanye i-rash ebomvu, ukubetha;Abanye baneentlungu zesisu, ukugabha kunye norhudo;Ukuthimla, ukutyibilika kwamathe, impumlo evuzayo, ukuphelelwa ngumphefumlo, ukubetha kwentliziyo ngokukhawuleza, i-conjunctival cyanosis.Ukukhutshwa okwandisiweyo ngenxa ye-bronchospasm kunye nobunzima bokuphefumla.Xa i-muscle egudileyo ye-organ spasms, kukho i-organ colic, i-suffocation, kunye nokufa.Ngokuqhelekileyo, ngakumbi iimpawu ezibukhali, i-prognosis imbi.

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Umgaqo-siseko wokufunyanwa

Umxholo we-cTIgE kwi-serum/plasma yafunyanwa ngokobungakanani nge-fluorescence immunochromatography.Imigaqo esisiseko:
Imigca ye-T kunye ne-C izotywa kwi-nitrate fiber membrane ngokulandelelanayo, kwaye imigca ye-T yafakwa kunye ne-antibody eyaqaphela ngokukodwa i-antigen ye-cTIgE.I-pad yatshizwa ngenye i-fluorescent nanomaterial ebhalwe i-antibody b, enokubona ngokuthe ngqo i-cTIgE.I-cTIgE yaqala ibotshelelwa kwi-nanomaterial ebhalwe i-antibody b ukwenza i-complex, kwaye emva koko ukuya kumaleko angaphezulu, i-antibody eyinkimbinkimbi kunye ne-T-line ibophelela ukwenza isangweji sesandwich.Xa ukukhanya okuvuyisayo kukhanya, i-nanomaterial ikhupha isignali ye-fluorescence.
Amandla omqondiso ahambelana ngokufanelekileyo kunye noxinaniso lwe-cTIgE kwisampuli.


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