I-Feline Leukemia Virus Antigen Quantitative Kit (Fluorescent Immunochromatography Assay of Rare Earth Nanocrystals) (FeLV Ag)

【 Injongo yovavanyo 】
I-feline leukemia virus (FeLV) yintsholongwane ye-retrovirus exhaphake kwihlabathi. Iikati ezisuleleke yintsholongwane zinomngcipheko omkhulu we-lymphoma kunye nezinye izidumbu; Intsholongwane inokubangela ukungahambi kakuhle kwe-coagulation okanye ezinye iziphazamiso zegazi ezifana ne-anemia evuselelayo / engabuyiyo; Kwakhona kunokukhokelela ekuweni kwamajoni omzimba, okukhokelela kwi-anemia ye-hemolytic, i-glomerulonephritis, kunye nezinye izifo.

【Umgaqo-siseko wokufunyanwa】
Iimveliso ziye zabalwa kwi-FeLV kwi-cat serum / plasma usebenzisa i-fluorescence immunochromatography. Umgaqo osisiseko: I-nitrocellulose membrane iphawulwe ngemigca ye-T kunye ne-C, ngokulandelanayo, kwaye umgca we-T uphawulwe nge-antibody A, eqaphela ngokukodwa i-antigen ye-FeLV. Iphedi yokubopha yafafazwa nge-anti-B ebhalwe ngenye i-nanomaterial ye-fluorescent ekwaziyo ukuqaphela ngokukodwa i-FeLV. I-FeLV kwisampulu yayibotshwe kuqala kwi-antibody B ebhalwe nge-nano-material ukuze yenze i-complex, kwaye emva koko yancitshiswa ukuya kwinqanaba eliphezulu. I-antibody ye-T-line eyinkimbinkimbi kunye ne-T-line yadityaniswa ukwenza isakhiwo sesandwich. Xa isibane esichukumisayo senziwa i-irradiated, i-nano-material yakhupha isignali ye-fluorescence, kwaye ukuqina komqondiso kuhambelana ngokufanelekileyo kunye noxinzelelo lwe-FeLV kwisampuli. Ngoko ke, ukufumanisa okuthembekileyo kunye nokusebenzayo kudlala indima efanelekileyo ekhokelayo ekukhuseleni, ukuxilongwa kunye nonyango.