【 Injongo yovavanyo 】
I-Cholyglycine (CG) yenye ye-conjugated cholic acid eyenziwe ngokudityaniswa kwe-cholic acid kunye ne-glycine.I-Glycocholic acid yeyona nxalenye ibalulekileyo ye-bile acid kwiserum ngexesha lokukhulelwa kade.Xa iiseli zesibindi zonakaliswe, ukunyuswa kweCG ngamaseli esibindi kuncipha, okubangele ukwanda komxholo weCG egazini.Kwi-cholestasis, ukukhutshwa kwe-cholic acid ngesibindi kuphazamisekile, kwaye umxholo weCG ubuyele kumjikelezo wegazi uyanda, okwandisa umxholo weCG egazini.
【Umgaqo-siseko wokufunyanwa】
Le mveliso isetyenziselwa ukufumanisa umthamo we-glycocholic acid (CG) egazini lezinja / iikati nge-fluorescence immunochromatography.Umgaqo osisiseko kukuba i-nitrocellulose membrane iphawulwe ngemigca ye-T kunye ne-C, kwaye umgca we-T uhlanganiswe ne-antigen a, eqaphela ngokukodwa i-antibody.i-antibody b ene-fluorescent nanomaterial enokwazi ngokuthe ngqo i-antigen A itshizwe kwiphedi yokubopha.I-antibody kwisampulu ibophelela kwi-antibody b nanomaterial ebhalwe ukuba yenze into entsonkothileyo, ethi emva koko iye phezulu.I-antigen eninzi kwisampulu ebotshelelwe yinkimbinkimbi, i-antibody encinci ye-fluorescent iya kubophelela kumgca we-T.Ukuqina kwalo mqondiso kuhambelana ngokungafaniyo noxinaniso lwe-antigen kwisampulu.
Ukusukela ekusekweni kwayo, umzi-mveliso wethu uye waphuhlisa iimveliso zodidi lwehlabathi ngokubambelela kumgaqo
yomgangatho kuqala.Iimveliso zethu ziye zafumana igama elihle kushishino kunye nokuthembeka okuxabisekileyo phakathi kwabathengi abatsha nabadala..