ITekhnoloji eNtsha yokutsalwa ngokupheleleyo kwe-nucleic acid kunye nomatshini wokubhaqa

Amandla amahlanu:
● Isixhobo esilungiselelwe ukutsalwa kwe-nucleic acid kunye nenyathelo elisulungekileyo
● Isixhobo esilungiselelwe ngemodyuli yokutsalwa kwe-ultrasonic
● Isixhobo similiselwe ngokuzenzekela ngokupheleleyo
● Isixhobo esilungiselelwe ngokwandiswa kobushushu obuguquguqukayo
● Isixhobo similiselwe kunye nekiti evalelweyo ngokupheleleyo

1.Ngaba ii-reagents zokubona i-nucleic acid kufuneka zikhutshwe kwaye zicocwe?
Umgaqo wokufumanisa i-nucleic acid umi ngolu hlobo lulandelayo: phantsi kwesenzo se-primer, i-DNA polymerase isetyenziselwa ukwenza i-chain reaction amplification kwi-template DNA / RNA (idinga ukuguqulelwa kwe-NA), kwaye emva koko isixa sesignali ye-fluorescent ekhutshiweyo ichongiwe ukumisela. ingaba isampuli iqulethe i-nucleic acid (i-DNA / i-RNA) ye-pathogen ukuba ibonwe.

I-1) Iisampulu ezingakhange zikhutshwe okanye zihlanjululwe zinokuthi ziqulethe izinto ezininzi ezichaphazela umphumo wokugqibela: i-nuclease (enokutshabalalisa i-nucleic acid ekujoliswe kuyo kwaye ibangele ubuxoki obungalunganga), i-protease (enokunciphisa i-DNA polymerase kwaye ibangele i-negative negative), isinyithi esinzima. ityuwa (ekhokelela ekusebenziseni i-synthase kwaye ibangele ubuxoki obubuxoki ), i-acidic kakhulu okanye i-alkaline kakhulu i-PH (enokubangela ukuba impendulo ingaphumeleli), i-RNA engaphelelanga (ekhokelela ekungaphumeleli kokubhala okubhaliweyo okungalunganga).

2) Ezinye iisampulu zinzima ukukhulisa ngokuthe ngqo: I-Gram-positive kunye nezinye iiparasite, ngenxa yeendonga zazo ezingqingqwa zeeseli kunye nezinye izakhiwo, ukuba azihambi nge-nucleic acid extraction kunye nenkqubo yokuhlanjululwa, ikhithi yokukhupha inokuthi ingaphumeleli kwizinto ezinjalo. iisampuli.

Ngoko ke, kucetyiswa ukuba ukhethe ikiti yokuvavanya okanye isixhobo esilungiselelwe inyathelo le-nucleic acid extraction.

2. Ukukhutshwa kweekhemikhali okanye i-physical ultrasonic fragmentation extraction?
Ngokuqhelekileyo, ukutsalwa kweekhemikhali kunokusetyenziswa kuninzi lonyango lwangaphambili kunye noCwangciso.Nangona kunjalo, kwiibhaktheriya ze-Gram-positive ezinodonga olungqingqwa kunye nezinye iiparasite, ikwayimeko yokuba ukutsalwa kweekhemikhali akukwazi ukufumana itemplates ze-nucleic acid esebenzayo, okukhokelela ekubhaqweni okungeyonyani.Ukongeza, ukutsalwa kweekhemikhali kaninzi kusebenzisa ii-agent ezinamandla, ukuba i-elution ayikho ngokucokisekileyo, kulula ukwazisa i-alkali eyomeleleyo kwinkqubo yokusabela, okukhokelela kwiziphumo ezingachanekanga.

I-Ultrasonic fragmentation isebenzisa ukutyunyuzwa komzimba, okuye kwasetyenziswa ngempumelelo yi-GeneXpert, ishishini elihamba phambili kumhlaba we-POCT ukuze kusetyenziswe abantu, kwaye kunenzuzo epheleleyo ekutsalweni kwe-nucleic acid kwiisampulu ezinzima (ezifana ne-Mycobacterium tuberculosis).

Ngoko ke, kucetyiswa ukuba ukhethe ikiti yokuvavanya okanye isixhobo esilungiselelwe inyathelo le-nucleic acid extraction.kwaye ilungile ukuba kukho imodyuli ye-ultrasonic extraction.

3. Imanuwali , iSemi-automatic kwaye izenzekela ngokupheleleyo?
Le yingxaki yeendleko zabasebenzi kunye nokusebenza ngokufanelekileyo.Okwangoku, izibhedlele zezilwanyana ezifuywayo ezingenabasebenzi abaneleyo, kunye ne-nucleic acid extraction kunye nokubonwa ngumsebenzi ofuna ubuchule kunye namava athile.Akukho mathandabuzo ukuba i-nucleic acid extraction kunye nomatshini wokufumanisa ngokuzenzekelayo lolona khetho lugqibeleleyo.

4. Ukwandisa ubushushu rhoqo okanye ukwandiswa kobushushu obuguquguqukayo?
I-amplification reaction yi-nucleic acid yokukhangela ikhonkco, kwaye iteknoloji yobuchwephesha ebandakanyekayo kule khonkco inzima.Ngokucacileyo, ii-enzymes zisetyenziselwa ukukhulisa i-nucleic acid.Kwinkqubo yokwandisa, isignali ye-fluorescence eyandisiweyo okanye isignali edibeneyo ye-fluorescence ifunyenwe.Ngokubanzi, okukhona umqondiso we-fluorescence ubonakala, kokukhona umxholo wejini ekujoliswe kuwo wesampulu.

Ukwandiswa kweqondo lobushushu rhoqo yi-nucleic acid amplification kwiqondo lobushushu elimiselweyo, ngeli lixa iqondo lobushushu eliguquguqukayo liyi-cyclic amplification ngokungqongqo ngokwe-denaturation-annealing-extension.Ixesha lokunyuswa kweqondo lokushisa eliqhubekayo liqhutywe, ngelixa ixesha lokunyusa ubushushu obuguquguqukayo lichaphazeleka kakhulu ngezinga lokunyuka kweqondo lokushisa kunye nokuwa kwesixhobo (okwangoku, abaninzi abavelisi baye bakwazi ukwenza imijikelezo ye-40 yokwandisa malunga nemizuzu engama-30).

Ukuba iimeko zaselabhoratri zilungile kwaye ucando lungqongqo, kuyavakala ukuba umahluko wokuchaneka phakathi kwezi zimbini awuyi kuba mkhulu.Nangona kunjalo, ukwandiswa kobushushu obuguquguqukayo kuya kudibanisa iimveliso ezininzi ze-nucleic acid ngexesha elifutshane.Kwiilabhoratri ezingenazo izowuni ezingqongqo kunye noqeqesho lwabasebenzi abaqeqeshiweyo, umngcipheko wokuvuza kwe-aerosol ye-nucleic acid uya kuba mkhulu, ubuxoki buvela nje xa kuvuza kusenzeka, kwaye kunzima kakhulu ukuyiphelisa.

Ukongeza, ukukhulisa ubushushu rhoqo kuqheleke ngakumbi kwi-amplification engacacisiweyo xa isampulu inzima (iqondo lobushushu elihambelanayo lisezantsi, kwaye ukuphakama kweqondo lobushushu liphezulu, ngcono ukucaciswa kokubophelela kweprimer).

Ngokubhekiselele kwitekhnoloji yangoku, ukukhulisa ubushushu obuguquguqukayo buthembeke ngakumbi.

5. Ungawunqanda njani umngcipheko wokuvuza kweemveliso zokwandisa i-nucleic acid?
Okwangoku, abavelisi abaninzi bakhetha uhlobo lwe-PCR ityhubhu ye-gland njengetyhubhu yokuphendula i-nucleic acid, etywinwe ngokukhuhlana, kunye ne-denaturation yeqondo lokushisa kwi-denaturation yokushisa okuguquguqukayo kwiqondo lokushisa eliguquguqukayo lokukhulisa i-PCR lifikelela kwi-90 degree.
I-Centigrade .Inkqubo ephindaphindiweyo yokwandisa kunye nokushisa kunye nokunciphisa ngokubanda ngumngeni omkhulu ekutywinweni kwetyhubhu ye-PCR, kwaye i-gland yodidi lwe-PCR ityhubhu kulula ukubangela ukuvuza.

Kungcono ukwamkela impendulo ngekhithi evalwe ngokupheleleyo/ityhubhu ukunqanda ukuvuza kwemveliso yokusabela.Iya kuba yinto egqibeleleyo ukuba kunokubakho umsebenzi wokuvala ikhithi etywinwe ngokupheleleyo yokutsalwa kunye nokubhaqwa kwe-nucleic acid.

Ke iNew Tech's entsha yokutsalwa kwe-nucleic acid kunye nomatshini wokubhaqa unokhetho olulungileyo olungentla.